The FluoroSpot assay at Creative Biolabs has been developed as an alteration of colorimetric substrates-based traditional enzymatic ELISpot detection systems. ELISpot is a good choice for the functional analysis of single immune cells, while FluoroSpot can be used for the functional analysis of 7 subsets of immune cells. The FluoroSpot is capable of detecting secreted cytokines or antibodies with a high level of sensitivity with the use of fluorochrome-conjugated detection antibodies.
Fig.1 6 General steps of FluoroSpot.
The most common cytokines investigated are IFN-ɣ, IL-2, IL-5, IL-10, IL-17, TNF, GM-CSF, Granzyme B, etc.
Fig.2 Most common cytokines investigated.
We also provide B cell FluoroSpot assays for:
Publication 1
Cells Used: Peripheral blood mononuclear cells (PBMCs)
Journal: Methods in molecular biology
IF: 0.368
Research Findings: PBMCs were stimulated with phorbol myristate acetate (PMA) and ionomycin in PVDF plates, and then, IFN-γ- and/or IL-2-producing cells were analyzed by a dual-color fluorospot assay. Results suggest the broad use of FluoroSpot assay for characterization of subgroups of T cells and polarization antigen-specific T cells.
Fig.3 Dual-color fluorospot for the detection of IL-2 and/or IFN-γ-producing cells.1
Publication 2
Cells Used: Peripheral blood mononuclear cells (PBMCs)
Journal: Journal for ImmunoTherapy of Cancer
IF: 10.9
Research Findings: PBMCs were analyzed for the secretion of IFN-ɣ, IL-17A, and IL-22 by FluoroSpot. 7 sub-populations, including 3 single secretors, 3 dual secretors, and 1 triple secretor, were identified by multi-level evaluation.
Fig.4 Overlay image of a triple color FluoroSpot well.2
Scientists at Creative Biolabs have first-class technology and offer comprehensive service in applying FluoroSpot assay.
For more detailed information about our FluoroSpot Service, please contact us immediately.
References
For Research Use Only | Not For Clinical Use