NGF (Nerve growth factor), also known as nerve growth factor (beta polypeptide), nerve growth factor beta subunit (NGFβ), beta-nerve growth factor (beta-NGF), NGFB or HSAN5, is a 27 kDa neurotrophic factor and a founding member of neurotrophins, a secreted growth factor family irresponsible for the growth, and developmental plasticity of neuronal populations in the vertebrate central and peripheral nervous system. NGF encodes a secreted protein of nerve growth factor beta of 241 amino acids and located on the chromosome 1p13.2. It acts by homodimerization and is incorporated into a large complex. NGF is essential for the development and survival of neurons, particularly those deliver pain, temperature, and touch sensations. This secreted protein functions and initiates signal pathways by binding two different receptors, NTRK1 or p75NTR, expressed on the surface of sensory neurons and other neurons. TNF (Tumor necrosis factor), also known as Cachectin, TNF-alpha, TNFA, TNFSF2 (Tumor necrosis factor ligand superfamily member 2), TNLG1F or DIF, is a 25.6 kDa proinflammatory cytokine that can stimulate the acute phase reaction and cause cell death or apoptosis. It has 233 amino acids encoded by the gene located at the chromosome 6p21.33. This factor is a member of the tumor necrosis factor superfamily (TNFSF), which can regulate many biological processes, including cell proliferation and differentiation, apoptosis, coagulation and lipid metabolism. It is a type of cytotoxin chiefly produced by activated macrophages, though can be expressed by other cells as well. This cytokine is involved in systemic inflammation and implicated in tumor regression, cachexia, and septic shock. And TNF functions through binding its receptors TNFRSF1A and TNFRSF1B.