Studies have shown that different from erythrocytes, several complement channels are required for the lysis of a nucleated mammalian cell. The propidium iodide (PI), which belongs to phenanthridine intercalators, is highly polar and water soluble to exclude reagents from passing through the cell membrane of living cells. When PI enters the cell, it will be tightly combined with the nucleus by being embedded in the DNA. Furthermore, the excitation and emission wavelengths of PI allow it to be detected by fluorescence microscopy replication or flow cytometry. Here, Creative Biolabs has developed the novel propidium iodide assay protocol to promote your research. Please note that our protocols are only for your reference.
Propidium Iodide Assay Protocol
Fig.1 Flow chart of the propidium iodide assay protocol. (Creative Biolabs)
Creative Biolabs has long-lasting expertise in the field of the human complement system. Our team of experts can assist you in designing the best study outline customized to meet the requirements of your discovery/development program. We value our client’s feedback and are committed to striving for one hundred percent client satisfaction. For customers' special project needs, we can also provide customized services based on standard protocols.
Services and Products at Creative Biolabs
If you are interested in our products and services, please do not hesitate to contact us for more information.
For Research Use Only.
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