Calcein AM refers to a cell-permeant dye that has been widely used for the determination of cell viability. In the living cells, non-fluorescent calcein AM is converted to green fluorescent calcein following hydrolysis of acetoxymethyl ester by intracellular esterases. In this case, the calcein release method can be served as a fluorescent equivalent of the 51Cr-release assay. What’s more, preloading cells with calcein does not affect cell proliferation and other functions. Here, Creative Biolabs has developed the novel calcein release assay protocol to promote your research. Please note that our protocols are only for your reference.
Calcein Release Assay Protocol
Fig.1 Flow chart of the calcein release assay protocol.
As a famous expert with a high reputation in the complement system market, Creative Biolabs describes a variety of protocols for our clients. Along with our complement-related products and services, we can work with you to create a timeline and plan for your operation. For customers' special project needs, we offer a variety of solutions to fit your specific research needs.
Services and Products at Creative Biolabs
If you are interested in our products and services, please do not hesitate to contact us for more information.
For Research Use Only.
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