Goat IL 18 ELISA Kit, Lot 21AO-1333 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Goat IL 18 in Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate.

The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IL18 in goat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

ELISA

IL 18

Goat

Colorimetric

Sandwich ELISA

Quantitative

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

This assay has high sensitivity and excellent specificity for detection of this index.

No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Pre-coated, ready to use 96-well strip plate
Plate sealer for 96 wells
Standard
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substrate
Stop Solution
Wash Buffer (30 x concentrate)
Instruction manual

1.Microplate reader with 450 ± 10nm filter.
2.Precision single or multi-channel pipettes and disposable tips.
3.Tubes for diluting samples.
4.Deionized or distilled water.
5.Absorbent paper for blotting the microtiter plate.
6.Container for Wash Solution.
7.0.01Mol/L (or 1x) Phosphate Buffered Saline(PBS), pH 7.0-7.2.

6.3 pg/mL

100 μL

1 - 4.5 h

Pre-coated

1.Add 100μL each of dilutions of standard, blank and samples into the appropriate wells, respectively. Cover with the Plate sealer. Incubate for 1 hour at 37 °C.
2.Remove the liquid of each well, don't wash.
3.Add 100μL of Detection Reagent A working solution to each well, cover the wells with the plate sealer and incubate for 1 hour at 37 °C.
4.Aspirate the solution and wash with 350μL of 1x Wash Solution to each well and let it sit for 1-2 minutes. Remove the remaining liquid from all wells completely by snapping the plate onto absorbent paper. Totally wash 3 times. Invert the plate and blot it against absorbent paper.
5.Add 100μL of Detection Reagent B working solution to each well, cover the wells with the plate sealer and incubate for 30 minutes at 37 °C.
6.Repeat the aspiration/wash process for total 5 times as conducted in step 4.
7.Add 90μL of Substrate Solution to each well. Cover with a new Plate sealer. Incubate for 10 - 20 minutes at 37 °C. Protect from light.
8.Add 50μL of Stop Solution to each well. Mix the liquid by tapping the side of the plate.
9.Remove any drop of water and fingerprint on the bottom of the plate. Then, run the microplate reader and conduct measurement at 450nm immediately.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.

4 °C,-20 °C

For unused kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon receipt while the others should be at 4 °C.
For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

12 months

For unused kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon receipt while the others should be at 4 °C.
For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

For Research Use only

Interleukin 18 (IL-18)

Igif; Il-18; IL-18; IGIF; ChIL-18; IL-1g; IL1F4; IL18; interleukin 18; Il18; IL18

Alternative name: IGIF, IL-1g, IL1F4, IL1-F4, Interferon-Gamma-Inducing Factor, Interleukin-1 Family Member 4, Iboctadekin, Interleukin-1 Gamma

100861190

Q3ZT29

Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome

1.Prepare all reagents, samples and standards.
2.Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C.
3.Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C.
4.Aspirate and wash 3 times.
5.Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C.
6.Aspirate and wash 5 times.
7.Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C.
8.Add 50μL Stop Solution. Read at 450nm immediately.