Human LFA-1 ELISA Kit, Lot 21AO-863 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P774
Product Type: ELISA Kit
Target: Integrin beta 2 (ITGB2)
Short Description
The kit is designed for in vitro quantitative measurement of Human LFA-1 in Plasma, Serum, Tissue Homogenate.
Description
For the quantitative determination of human integrin β2 (ITG β2/CD11+CD18) concentrations in serum, plasma, tissue homogenates.
Applications
ELISA
Application Notes
Optimal working dilution should be determined by the investigator.
Target
Integrin beta 2 (ITGB2)
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Plasma, Serum, Tissue Homogenate
Specificity
This assay has high sensitivity and excellent specificity for detection of human ITG β2/CD11+CD18. No significant cross-reactivity or interference between human ITG β2/CD11+CD18 and analogues was observed.
Components
Assay plate
Standard
HRP-avidin (100 x concentrate)
Biotin-antibody (100 x concentrate)
Sample Diluent
HRP-avidin Diluent
Biotin-antibody Diluent
Wash Buffer (25 x concentrate)
TMB Substrate
Stop Solution
Adhesive Strip
Sensitivity
3.9 pg/mL
Sample Volume
100 μL
Assay Time
1 - 4.5 h
Plate
Pre-coated
Reagent Preparation
Biotin-antibody (1×) - Centrifuge the vial before opening.
HRP-avidin (1×) - Centrifuge the vial before opening.
Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved.
Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.
Assay Precision
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Storage
4 °C,-20 °C
Storage Comment
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8°C. If Biotin-antibody doesn't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.
Expiry Date
6 months
Note
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.
Restrictions
For Research Use only
Alternative Name
integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Synonyms
CD18; LAD; LCAMB; LFA-1; MAC-1; MF17; MFI7; 2E6; AI528527; Cd18; Lfa1; ITGB2; itgb2; itgb2-a; itgb2-b; integrin subunit beta 2; integrin beta 2; integrin subunit beta 2 S homeolog; ITGB2; Itgb2; itgb2.S
Background
Abbreviation: ITGB2
Alias: CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7, OTTHUMP00000115279|OTTHUMP00000115281|OTTHUMP00000115282|cell surface adhesion glycoprotein LFA-1/CR3/P150,959 beta subunit precursor)|complement receptor
Gene ID
3683
UniProt
P20701
Pathways
NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
For Research Use Only | Not For Clinical Use
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