K562 In Vitro Cell Membrane Integrity-based Apoptosis Assay (FCM)

This assay is to provide K562-based In Vitro Cell Membrane Integrity-based Apoptosis Assay (FCM) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

K562

Human

K562 cells are of the erythroleukemia type, and the cell line is derived from a 53-year-old female chronic myelogenous leukemia patient in blast crisis. The cells are non-adherent and rounded, are positive for the bcr:abl fusion gene, and bear some proteomic resemblance to both undifferentiated granulocytes and erythrocytes.

K-562

Chronic Myeloid Leukemia; CML

Oncology

In Vitro Cell Membrane Integrity-based Apoptosis Assay (FCM)

K562-cell based In Vitro Cell Membrane Integrity-based Apoptosis Assay (FCM)

FCM is one of the best and accurate automated tecqniques used for the quantification of apoptosis. FCM has been widely used in cancer drug discovery approaches to detect apoptosis. FCM can be used to monitor integrity of cancer cell membranes. Dyes such as PI, fluorescein diacetate, carboxyfluorescein diacetate and TO-PRO-3 are commonly used examples for this purpose in FCM.

Detection of Apoptosis Assays

Apoptosis (programmed cell death) plays a vital role in embryonic development, homeostasis, functioning of immune system and wound repair. The ability to evade induction of apoptosis has been used by cancer cells to survive against host defense mechanisms. The molecular mechanisms involved in cancer cell apoptosis have been well documented and it involves certain biochemical events such as DNA fragmentation, chromatin condensation, cell organelle degradation and protein cleavage, etc. The extrinsic and intrinsic (mitochondrial) pathways are the two major pathways involved in apoptosis. With the available techniques and assays, a number of apoptosis inducing agents (natural compounds, synthetic compounds, nano-formulations, peptides and enzymes) in many cancer cells have been identified. Selection of an assay for apoptosis detection is based on factors such as apoptotic pathway, nature of drug, cell type being used and the method of analysis.

Flow Cytometry to Detect Apoptosis Assays

Flow Cytometry Assay