The LipidFlux™ Malate Assay Kit tracks intermediates in the TCA cycle and malate-aspartate shuttle, supporting research into cellular respiration and metabolic adaptation. The NADH-coupled system provides the sensitivity needed to investigate mitochondrial bioenergetics in various tissue types. This assay aids in understanding how metabolic modulators influence energy production and is valuable for studying diseases associated with mitochondrial dysfunction.
Features
NADH-coupled system tracks energy metabolism; Supports investigation of mitochondrial bioenergetics and malate-aspartate shuttle activity.
Process Relevance
Bioenergetics Research
Application Stage
Basic Research
Applications
Plant physiology and TCA cycle research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565 nm
Sample Type
Malate in food, juice, beverage, and other agricultural products
Research Areas
Metabolic Disease; Bioenergetics
Size
100 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 15 min
LOD
0.02 mM
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Malate is an intermediate in the TCA cycle and a component of the malate-aspartate shuttle. It plays a significant role in transporting NADH reducing equivalents across the mitochondrial membrane for ATP production.