We perform reliable competitive binding assays to determine your compound's binding affinity for the CCR2 receptor, delivering accurate IC50 and Kd values.
CCR2 Assay Portfolio Service
Creative Biolabs offers a specialized C-C chemokine receptor type 2 (CCR2) assay portfolio service to support targeted cancer therapy and diagnosis. We utilize advanced technologies and experienced scientists to precisely assess the interplay between the chemokine CCL2 and its receptor CCR2. These molecules are recognized as key mediators of cancer-related inflammation, promoting tumor progression, metastasis, and immunosuppression. Our comprehensive service provides the critical data needed to characterize this signaling axis, advancing CCR2 as a promising therapeutic target in oncology.
Background What We Can Offer Publication Why Choose Us FAQs Customer Review Related Services Contact Us
Role of Chemokines in Tumors
Chemokines are small, secreted cytokines that play a vital role in immunity and inflammation, which is a key component of the tumor microenvironment.
- Pro- or Anti-tumoral Roles: Inflammatory CXC and CC chemokines can have pro- or anti-tumoral roles by recruiting neutrophils and monocytes to the tumor site.
- Tumor Angiogenesis and Metastasis: Chemokines and their receptors can promote tumor angiogenesis, which is essential for tumor growth and metastatic spread.
- Cancer Cell Proliferation and Migration: Chemokine receptors expressed on cancer cells can directly promote their proliferation by activating signaling pathways and can also promote their migration to metastatic sites.
- Cancer Therapy: Targeting chemokines and their receptors represents a concrete approach against cancer, as they are involved in various aspects of cancer biology.
Fig.1 The role of chemokine receptor inhibition in cancer. 1, 3
Our Assays: From Ligand Binding to Functional Response
Our comprehensive service portfolio includes a wide range of assay types, using both biochemical and cell-based methods to thoroughly characterize compounds that target CCR2. This integrated strategy provides a complete picture of your drug candidate's mechanism of action.
Receptor Binding Assays
Calcium Flux Assays
Using high-throughput fluorescent imaging, we track real-time intracellular calcium changes—enabling efficient screening of antagonist activity.
Cell Migration Assays
Through transwell and wound-healing models, we evaluate monocyte migration in response to CCL2 gradients, offering functional insight into chemotactic inhibition.
Protein Detection & Quantification
We offer highly sensitive ELISA kits to measure CCR2 protein levels, and complement this with Western blotting for expression analysis and validation.
Cell Surface Receptor Analysis
Using flow cytometry, we profile CCR2 expression across different cell populations, supporting target engagement and selectivity studies.
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Publication
This review explores the critical role of the CCL2/CCR2 chemokine signaling axis in cancer pathogenesis, particularly in prostate cancer (PCa). This ligand-receptor pair is crucial for recruiting immunosuppressive myeloid cells, such as tumor-associated macrophages (TAMs) and myeloid-derived suppressor cells (MDSCs), into the tumor microenvironment. This recruitment promotes a pro-tumorigenic niche that fosters inflammation, angiogenesis, and metastasis. The review also highlights how obesity-induced inflammation can increase CCL2 levels and accelerate tumor growth. The authors' research shows that pharmacologically inhibiting CCR2 significantly reduces tumor growth and cell migration. The review concludes that the CCL2/CCR2 axis is a promising therapeutic target for treating prostate and other obesity-associated cancers.
Fig.2 Crosstalk between tumor cells and pro-inflammatory factors in the tumor microenvironment. 2, 3
Why Choose Us?
Creative Biolabs offers a distinct advantage rooted in our scientific expertise, advanced technology, and unwavering commitment to quality. Our state-of-the-art high-throughput facilities are designed for speed and reliability, while our stringent quality control measures guarantee reproducible and reliable data. We are not just a service provider; we are a partner in your research journey, offering bespoke solutions and expert guidance to help you overcome your most challenging obstacles. The CCL2/CCR2 axis is a complex target, and our specialized expertise ensures your research is grounded in the most current scientific understanding.
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FAQs
Q1: What cell lines do you use for your functional assays?
A1: We use a variety of cell lines that endogenously express CCR2 or are engineered to express the receptor at a high level. We select the most appropriate cell line based on the assay type and your specific project goals.
Q2: Can your assays be used to screen for both CCR2 antagonists and agonists
A2: Yes, our portfolio includes a wide range of assays designed to identify and characterize both antagonists and agonists. For antagonists, we can measure their ability to block CCL2-induced responses, while for agonists, we can measure their ability to mimic those responses.
Q3: What is the minimum amount of compound I need to provide for a screening project
A3: The required amount of compound depends on the specific assays and the number of compounds in your library. We will provide detailed guidance on the necessary quantities during the initial project consultation.
Customer Review
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Highly confident in our data
Using Creative Biolabs' CCR2 assay portfolio service in our research has significantly improved our ability to confidently validate the binding affinity of our compounds. - Aan T* -
Accelerated our screening
The high-throughput calcium flux assays from Creative Biolabs were a game-changer for our large-scale screening project. The speed and real-time data allowed us to rapidly filter our compound library and focus on the most promising antagonists. - Pl R*
Related Services
To further support your research, we offer a range of complementary services that may be relevant to your CCR2-related project.
Strategies for Engineering Cancer Cells
Creative Biolabs has developed robust in vitro and in vivo platforms to provide custom-engineering cancer cell-based immunotherapy strategies. Our scientists offer valuable experience to help clients shorten the preclinical study journey.
Learn More →HER2 Cell Panel Screening Service
Creative Biolabs provides comprehensive HER2 cell panel screening services for preclinical drug evaluation. The company utilizes cutting-edge platforms for both 2D and 3D cell panel screenings to ensure robust data that mimics in vivo environments.
Learn More →Contact Us
The CCL2/CCR2 axis remains a promising target for next-generation immunotherapies. By partnering with Creative Biolabs, you gain access to a world-class team and a versatile assay portfolio that will accelerate your research and bring you one step closer to a therapeutic breakthrough.
Contact Our Team for More Information and to Discuss Your Project.
References
- Mollica Poeta, Valeria, et al. "Chemokines and chemokine receptors: new targets for cancer immunotherapy." Frontiers in immunology 10 (2019): 379. https://doi.org/10.3389/fimmu.2019.00379
- Hao, Qiongyu, Jaydutt V. Vadgama, and Piwen Wang. "CCL2/CCR2 signaling in cancer pathogenesis." Cell communication and signaling 18.1 (2020): 82. https://doi.org/10.1186/s12964-020-00589-8
- Distributed under Open Access license CC BY 4.0, without modification.
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