Lysine Acetyltransferase (KAT) Assay Service
At Creative Biolabs, our KAT Assay Service provides a reliable platform for assessing the enzymatic activity of KAT family members and their roles in immune-modulated cancer pathways. Our service supports drug discovery, target validation, and mechanistic research in epigenetic immunology.
Background
Lysine acetyltransferases (KATs), also known as histone acetyltransferases (HATs), catalyze the transfer of acetyl groups to lysine residues on histones and non-histone proteins. This epigenetic modification opens chromatin for transcriptional activation and regulates numerous immune-related processes, including cytokine production, antigen processing, and T cell differentiation. In the tumor microenvironment, dysregulated KAT activity may enhance tumor immune evasion by altering gene expression involved in immune recognition or by modulating immune cell phenotype.
Featured Services at Creative Biolabs
We offer robust solutions to detect, quantify, and characterize the functional activities of major KAT family enzymes, including CBP/p300, GCN5, PCAF, and Tip60:
Fluorometric Acetyltransferase Activity Assay
Quantitative detection of acetyl group transfer to peptide or protein substrates using fluorescent detection reagents
Radioisotope-Based Acetylation Assay
High-sensitivity incorporation of ³H-acetyl-CoA onto lysine residues of synthetic or native substrates
Western Blot & ELISA for Acetylation Detection
Antibody-based readouts of global or site-specific histone/non-histone protein acetylation (e.g., Ac-H3K27, Ac-p53)
Chromatin Context Assays
Use of reconstituted nucleosomes or isolated chromatin for functional relevance in immune gene activation studies
Cell-Based Acetylation Monitoring
Measurement of transcriptional outcomes of KAT inhibition on genes related to antigen presentation, interferon response, and T cell co-stimulation
High-Throughput Capability
Assays adaptable to 96/384-well screening for epigenetic modulator libraries with validated controls
Workflow
Highlights
- Immunoepigenetics Emphasis – Focused on KAT impact on immune gene transcription and tumor–immune dynamics
- Comprehensive Enzyme Coverage – Includes CBP, p300, GCN5, PCAF, Tip60, and others relevant to immune signaling
- Flexible Readout Formats – Choose from fluorescence, radiolabel, ELISA, or western blot depending on sensitivity needs
- Chromatin-Relevant Contexts – Assays performed on chromatin templates or in intact cells for physiological relevance
- Ideal for Screening & Mechanistic Insight – Supports early discovery and downstream validation phases
FAQs
Q1: Can your platform differentiate between specific KAT enzymes?
A1: Yes. We offer both isoform-specific assays and selective inhibitors to distinguish between CBP/p300, GCN5, and Tip60 activities.
Q2: Are your assays compatible with immune cells or co-culture models?
A2: Yes. We can measure KAT activity or acetylation effects in tumor-infiltrating immune cells, dendritic cells, or macrophage systems.
Q3: How do you detect changes in immune-related gene expression following KAT modulation?
A3: We provide downstream transcriptional profiling using qPCR arrays for genes involved in MHC expression, checkpoint regulation, and interferon signaling.
Q4: Do you support kinetic analysis for hit compound validation?
A4: Yes. We can deliver IC₅₀, Km, and Vmax data using multiple detection platforms to characterize compound–enzyme interactions.
Q5: Can KAT assays be applied to screen immune-enhancing epigenetic drugs?
A5: Absolutely. Our platform is optimized for screening libraries targeting immune-related transcriptional activation via histone acetylation.
Partner with Us for Advanced Epigenetic Profiling in Immuno-Oncology
At Creative Biolabs, we help scientists explore how KAT enzymes regulate immune responses within the tumor microenvironment. Our tailored assay solutions are designed to uncover novel immune-epigenetic interactions and accelerate immunomodulator discovery.
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