ModifiSite™ α-Amylase Inhibitor Screening Kit

CAT#: ITS-1125-WB24
Product Type: Assay Kit
Species: All Species
Target: Human salivary and pancreatic α-amylase enzymes (EC 3.2.1.1).
Brand: ModifiSite
Development Stage: Research-Grade Protein-Based Assay Development
Short Description
Screens for α-amylase inhibitors using a fluorescence polarization (FP) method.
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ α-Amylase Inhibitor Screening Kit utilizes advanced fluorescence polarization technology to identify potential drug candidates for metabolic disorders. This robust, high-throughput system eliminates matrix interferences, providing reliable kinetic data to evaluate inhibitor efficacy. It is an essential tool for streamlining lead discovery and optimizing therapeutic strategies targeting carbohydrate digestion.
Features
Unrivaled Sensitivity: Detects subtle inhibitory effects with high precision, ensuring the identification of low-affinity leads during early-stage drug screening.
Robust Assay Format: Utilizes a homogeneous "mix-incubate-measure" workflow, eliminating complex wash steps and maximizing experimental efficiency and reproducibility.
Superior Chemical Specificity: Minimizes background interference through optimized substrate chemistry, yielding high Z'-factors suitable for automated high-throughput screening environments.
Ready-to-Use Components: Includes pre-validated human α-amylase and specific control inhibitors, streamlining protocol implementation and ensuring standard-compliant comparative analysis.
Process Relevance
Optimizing α-amylase inhibition kinetics to validate therapeutic efficacy and ensure consistent enzymatic process control in drug manufacturing.
Application Stage
Targeted for hit-to-lead drug discovery, pre-clinical metabolic profiling, and in-process quality assessment of natural bioactive compounds.
Applications
This kit facilitates high-throughput screening of synthetic compound libraries, evaluation of natural plant extracts, and kinetic characterization of reversible or irreversible competitive enzymatic inhibitors.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Human salivary and pancreatic α-amylase enzymes (EC 3.2.1.1).
Detection Method
Fluorescence Detection (Ex 485 nm / Em 520 nm)
Sample Type
Chemical compounds
Research Areas
Diabetes mellitus, obesity management, gastrointestinal physiology, clinical nutrition, agricultural pest resistance, etc.
Size
400 tests
Data Output Type
Quantitative determination of percentage inhibition and IC50 values via kinetic or endpoint absorbance monitoring.
Assay Time
40 min
Species
All Species
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
α-Amylase is a critical endoglycosidase that initiates the breakdown of complex dietary starches into absorbable monosaccharides. Therapeutic modulation of this enzyme is essential for managing postprandial glycemic spikes in type 2 diabetes.
Pathways
This enzyme operates within the primary carbohydrate metabolic pathway, hydrolyzing α-1,4-glycosidic bonds upstream of α-glucosidase. Its inhibition prevents rapid glucose entry into the bloodstream, directly impacting insulin-mediated glucose homeostasis.
For Research Use Only | Not For Clinical Use
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