Poly(ADP-ribose) Polymerase (PARP) Assay Service
Creative Biolabs offers comprehensive PARP Assay Services tailored to investigate enzyme kinetics, inhibitor profiling, and immune-related functions of PARP enzymes, supporting immuno-oncology research and drug discovery.
Background
Poly(ADP-ribose) polymerases (PARPs) are key regulators of DNA damage response (DDR) and post-translational modification through poly-ADP-ribosylation (PARylation). Among the 17 PARP family members, PARP1 and PARP2 play prominent roles in sensing DNA strand breaks and orchestrating repair. Beyond genome maintenance, PARPs have emerged as critical immunomodulators by influencing STING signaling, immunogenic cell death (ICD), and tumor antigenicity. Inhibiting PARPs can enhance the efficacy of immunotherapy through cGAS-STING pathway activation and PD-L1 expression modulation.
Featured Services at Creative Biolabs
We provide diverse biochemical and cellular tools for evaluating PARP functions in the context of tumor–immune interactions:
Colorimetric PARP Activity Assay
Quantitative analysis of NAD⁺ conversion to poly(ADP-ribose) using antibody-based detection of PAR chains
Fluorescence-Based Assays
Real-time monitoring of PARP1/2 activity using labeled NAD⁺ analogs or biotinylated PAR substrates
Radiolabeled Substrate Assays
High-sensitivity detection of [³²P]-NAD⁺ incorporation onto nuclear acceptor proteins
Cell-Based PARP Activity Profiling
Quantification of PAR levels in tumor or immune cells post-DNA damage induction, including immunogenic stress conditions
STING Pathway Integration
Evaluation of cGAS-STING activation following PARP inhibition or hyperactivation in tumor-immune co-culture models
High-Throughput Inhibitor Screening
Screening libraries against PARP1, PARP2, or tankyrase isoforms in 96/384-well formats with IC₅₀ reporting
Workflow
Highlights
- Immune-Focused Context – Supports studies on PARP-induced STING activation and ICD in cancer immunology
- Multiplex Detection Systems – Fluorescence, radiolabeling, and colorimetric readouts to suit sensitivity and throughput needs
- Isoform Specificity – Capable of evaluating PARP1/2 as well as tankyrases in Wnt and immune-related signaling
- Functional Relevance – Assays can be performed in cells undergoing DNA damage, oxidative stress, or immunogenic treatments
- Custom Co-Culture Models – Integrate tumor cells with immune cells to observe PARP inhibition impact on immune activation
FAQs
Q1: Do you offer assays specific for PARP1 vs. PARP2?
A1: Yes, we utilize isoform-selective substrates and inhibitors to distinguish PARP1 from PARP2 or tankyrase activity.
Q2: Can your platform assess immune outcomes of PARP inhibition?
A2: Absolutely. We integrate cGAS-STING activation, cytokine release assays, and PD-L1 expression analysis post-PARP modulation.
Q3: What is the sensitivity range of your enzymatic assays?
A3: Our radiolabeled and fluorescence-based systems can detect PARP activity in the nanomolar range with high reproducibility.
Q4: Are your assays compatible with DNA-damaging agents or immunogenic stimuli?
A4: Yes. We routinely incorporate etoposide, doxorubicin, or ionizing radiation to model damage-induced PARylation and immune priming.
Q5: Do you support screening of PARP inhibitors in immuno-relevant cell lines?
A5: We offer screening services using tumor and immune cell lines, including checkpoint-relevant backgrounds (e.g., PD-L1+, STING+ models).
Advance Your Immuno-Oncology Pipeline with High-Resolution PARP Profiling
Creative Biolabs empowers your research into DNA damage-induced immune responses through precise PARP activity measurements and mechanistic insights. Partner with us for tailored assay development and data-driven discovery.
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