At Creative Biolabs, we offer robust SIRT Assay Services to support the characterization of sirtuin enzymatic activity, inhibitor screening, and immune-functional pathway analysis—empowering immune-oncology research and therapeutic development.
Background
Sirtuins (SIRTs) are a family of NAD⁺-dependent deacetylases and ADP-ribosyltransferases that orchestrate epigenetic regulation, DNA repair, metabolism, and immune signaling. In oncology, SIRTs (particularly SIRT1, SIRT2, and SIRT6) are increasingly recognized for their roles in immune evasion, T cell metabolism, tumor-associated macrophage (TAM) polarization, and PD-L1 regulation. Their modulation presents a strategic opportunity to reshape the tumor immune microenvironment (TIME).
Featured Services at Creative Biolabs
Our SIRT assay services span multiple platforms, allowing precise interrogation of NAD⁺-dependent activity and immuno-functional consequences:
Fluorometric SIRT Deacetylase Assay
Uses acetylated lysine fluorogenic substrates to quantify activity of SIRT1–SIRT7 in real time
HPLC-Based Substrate Profiling
High-resolution analysis of deacetylation kinetics and substrate specificity under physiological or hypoxic conditions
SIRT-Mediated Immunoregulation Studies
Includes assessment of SIRT1/6 roles in NF-κB signaling, PD-L1 expression, or interferon pathway regulation
NAD⁺/NADH Dependency Mapping
Correlate metabolic states with sirtuin activation to model immunosuppressive vs. immunostimulatory phenotypes
High-Throughput Inhibitor Screening
Platform for screening small molecules, peptides, or biologics targeting sirtuins, with readouts tied to immune gene expression
Cellular Models with Immune Relevance
Tumor cells co-cultured with immune cells to explore SIRT-induced T cell exhaustion or macrophage polarization
Workflow
Highlights
Isoform-Specific Assays – SIRT1–SIRT7 profiling available across multiple biological contexts
Immunometabolic Insight – Dissect links between sirtuin activity and T cell fate, macrophage activation, and immune escape
Multi-Readout Detection – Fluorescence, HPLC, and gene expression panels available for complete activity tracking
Integration with NAD⁺ Metabolism – Supports metabolic rewiring studies in tumor immune modulation
Custom Assay Design – Tailored platforms for inhibitor discovery, pathway interaction, or TIME remodeling
FAQs
Q1: Which SIRTs are most relevant to tumor immunity?
A1: SIRT1, SIRT2, and SIRT6 are heavily implicated in immune regulation, including PD-L1 expression, cytokine signaling, and immune suppression.
Q2: Can you assess sirtuin activity under different metabolic conditions?
A2: Yes. We can model glucose restriction, hypoxia, or NAD⁺/NADH alterations to mimic tumor microenvironmental stress.
Q3: Do you support co-culture models for SIRT-immune interaction studies?
A3: Absolutely. We offer tumor + T cell or macrophage co-cultures to observe SIRT effects on immune cell phenotype and function.
Q4: What kinds of SIRT inhibitors can you test?
A4: We test small molecules, peptides, or natural compounds across SIRT1–SIRT7 using fluorometric or gene-expression-linked platforms.
Q5: Are immune gene expression profiles included in analysis?
A5: Yes. We offer optional transcriptomic profiling to correlate SIRT modulation with immune-related gene changes.
Explore the Immunological Dimensions of Sirtuin Enzymes with Precision Assays
Creative Biolabs delivers expert-level SIRT assay platforms for researchers investigating immune metabolism, tumor suppression, and checkpoint regulation. Let us accelerate your discoveries in the immuno-oncology space.
