An Overview of TRP Channels

The transient receptor potential (TRP) channels are a diverse group of ion channels found in the cell membranes of various cell types. They play an important role in numerous physiological processes, including sensory perception, temperature regulation, and pain sensation. TRP channels are activated by a wide range of stimuli, such as temperature changes, mechanical force, light, and chemical signals like pheromones or endogenous molecules. There are several families of TRP channels (TRPC, TRPV, TRPM, TRPA, TRPP, TRPML, and TRPN), each characterized by their distinct activation mechanisms and physiological roles. TRP channels have been implicated in many disease states, including chronic pain, neurodegenerative diseases, and cancer, making them promising targets for therapeutic research and drug development. Due to their wide range of functions and involvement in various diseases, TRP channels are an ongoing research focus for developing therapeutic drugs targeting specific TRP channels for conditions like pain, inflammation, and metabolic diseases.

Fig.1 Human TRP channels.¹

TRP Channel Assay Service

At Creative Biolabs, we offer a comprehensive TRP channel assay service designed to support therapeutic antibody and protein development. Our service is specifically tailored for the high-throughput screening and characterization of TRP channel activity, ensuring that researchers and developers can rapidly assess the functional effects of potential therapeutic candidates. Our TRP channel assay service uses state-of-the-art technologies to evaluate the modulation of various TRP channel types, including TRPV1, TRPV4, TRPA1, and others. By targeting these channels, therapeutic antibodies and proteins can be developed to regulate TRP channel activity and address various diseases at the molecular level.

Key Features of Our TRP Channel Assay Service

• Comprehensive Assays

Using advanced technologies like fluorometric imaging assay, manual/automated patch-clamp electrophysiology, and calcium flux assays, we provide real-time monitoring of TRP channel activity. This enables detailed kinetic analysis of channel activation or inhibition, which is essential for understanding the pharmacodynamics of potential therapeutic agents.

• Wide Range of TRP Channels Tested

We offer assays for a broad spectrum of TRP channels, including TRPV, TRPA, TRPM, and others. Our service covers both canonical and non-canonical TRP channels that are involved in different cellular responses.

• High Throughput Screening

Our service is available for high-throughput screening, enabling the fast assessment of large compound libraries, antibodies, or proteins. This is especially valuable in drug discovery and early-stage development.

At Creative Biolabs, we offer a comprehensive TRP channel assay service to support the development of therapeutic antibodies and proteins aimed at modulating TRP channel activity. Whether you are working on designing novel therapeutics or evaluating existing compounds, our service can be tailored to meet the unique needs of your research.

Applications

• Pain and Inflammation Research
  TRP channels such as TRPV1 and TRPA1 are key mediators in nociception and inflammatory responses. Our TRP channel assay enables researchers to identify and characterize compounds or antibodies that modulate these channels, accelerating the development of novel therapeutics for chronic pain and inflammatory conditions.
• Neurological and Neurodegenerative Disorders
  Given the role of TRP channels in sensory transduction and neuronal signaling, our assays are valuable for exploring therapeutic strategies targeting TRP channels in neurodegenerative diseases such as Alzheimer's, Parkinson's, and multiple sclerosis, where TRP dysregulation contributes to disease progression.
• Oncology Drug Discovery
  Certain TRP channels are upregulated in tumor cells and involved in cancer cell proliferation, migration, and survival. Our assay platform supports the screening of biologics and small molecules targeting TRP channels like TRPM8 and TRPC6, aiding oncology research and therapeutic development.
• Metabolic and Cardiovascular Disease Studies
  TRP channels regulate calcium homeostasis and vascular tone, linking them to metabolic syndromes and cardiovascular diseases. Our comprehensive assay service facilitates the discovery of TRP-targeting agents with potential therapeutic benefits in conditions like hypertension, atherosclerosis, and diabetes.

For more information on how our TRP channel assay service can support your therapeutic development programs, please contact Creative Biolabs today.

Reference

1. Méndez-Reséndiz, Karina Angélica, et al. "Steroids and TRP channels: a close relationship." International Journal of Molecular Sciences 21.11 (2020): 3819. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only | Not For Clinical Use