As a pioneer and the undisputed global leader in complementary research, Creative Biolabs offers a series of products and services for our clients all over the world. Anaphylatoxins, also known as complement peptides, are fragments produced as parts of the activation of the complement system. C3a is an important anaphylatoxin that together with C5a contributes to the initiation of the adaptive immune response in several ways. Here, we describe the anaphylatoxin C3a purification protocol to promote your research. Please note that our protocols are only for your reference.
Anaphylatoxin C3a Purification
Fig.1 Flow chart of anaphylatoxin C3a purification. (Creative Biolabs)
Published Data
Recombinant human C3a and its desArg variant were produced in Escherichia coli and purified using a sequential chromatographic protocol. Initially, cells were subjected to Ni2±NTA affinity purification in two stages, ensuring the capture of the target proteins, followed by an ion-exchange step that further refined purity. On the cation-exchange column, C3a and C3a desArg emerged as single, monodisperse peaks, eluting at 380 mM and 330 mM NaCl respectively, reflecting their distinct isoelectric properties. The overall yield was approximately 0.5–0.7 mg per liter of bacterial culture. These data confirm protocol consistency.
Equipped with world-leading technology platforms and professional scientific staff, Creative Biolabs is capable of meeting the specific need of each customer with the best project design. Now we can provide a range of complementary products and complement assay services, including therapeutic antibodies, serine protease inhibitors, soluble complement regulators, complement component inhibitors, etc.
To learn more details, you can get access with the following links:
-
Complement Products
-
Complement Test Services
If you are interested in our products and services, please do not hesitate to contact us for more information.
For Research Use Only.
