C1-inhibitor (C1-INH) is defined as a protease inhibitor that plays a crucial role in the activation of the complement pathway by inhibiting the activated C1r and C1s. C1-INH is a crucial regulator that suppresses the complement system to prevent spontaneous activation. In addition, the main functions of C1-INH also include inhibiting the enzymes related to coagulation, clot lysis and kinin production. Creative Biolabs is a recognized expert in the field of complement research. More than 10 years of experience in complement research and a comprehensive range of validated analytical methods ensure the highest cost-effective products and services for our global customers.
C1-INH Purification from Human Plasma Protocol
C1-INH is considered an acute phase protein that circulates in plasma at a concentration of 0.25 g/L. Concentrations of C1-INH can be elevated approximately 2-fold during inflammation. C1-INH can be purified from human plasma, and plasma-derived C1-INH has been used for many years for the therapy of patients with C1-INH deficiency. Here, we provide a simple protocol for small scale purification of C1-INH.
Fig.1 Flow chart of C1-INH purification protocol. (Creative Biolabs)
As a world-leading biotechnology company, Creative Biolabs has been at the forefront of complement research. The availability of the most comprehensive complement testing systems and unrivaled experience working with clients worldwide make us the first choice for complement research. Our extensive complement products such as proteins, kits, and vectors are suitable for many different research purposes. In addition, a variety of services, such as complement assay services, compstatin development services, complement activity and functional detection analysis services are available. Advanced analysis technology provides a favorable guarantee for our comprehensive analysis services.
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Please note that our protocols are only for your reference.
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