Creative Biolabs has been focusing on the research of the complement system for many years and can provide a variety of products and services to our customers. Drug-induced hemolysis is a serious toxicity tendency, which can be divided into toxic hemolysis and allergic hemolysis by different mechanisms. It has been proved that the classical pathway hemolysis assay can be applied to assess the functional activity of recombinant complement C3. Here, we describe the C3 analysis by hemolysis assay protocol to promote your research. Please note that our protocols are only for your reference.
C3 Analysis by Hemolysis Assay
Fig.1 Flow chart of C3 analysis by hemolysis assay protocol. (Creative Biolabs)
Published Data
Fig.2 Comparative hemolytic activity of various C3 formulations assessed via a classical complement pathway assay.1
The hemolytic potential of various C3 preparations was evaluated using a classical pathway hemolysis assay. The lysis of 0.05% IgM sensitized erythrocytes, i.e., the hemolytic potential of C3 was evaluated by incubating cells in C3-depleted serum supplemented with native C3, C3b, pH 11-treated C3, or various freeze/thaw C3 preparations. Specifically, one preparation comprised precipitated and native C3 subjected to five cycles between –20°C and room temperature, while another involved C3 cycled five times from –80°C to 37°C. Complete lysis was achieved by exposing cells to pure water, and the extent of hemolysis was quantified by measuring absorbance at 414 nm. These findings influence investigations into the structural configuration and functional properties of C3.
Depending on novel research protocols and tools, Creative Biolabs is capable of meeting the specific need of each customer with the best project design. Now we can provide a range of complementary products and complement assay services, including therapeutic antibodies, serine protease inhibitors, soluble complement regulators, complement component inhibitors, etc.
To learn more details, you can get access with the following links:
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Complement Products
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Complement Test Services
If you are interested in our products and services, please do not hesitate to contact us for more information.
Reference
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Adler, Anna, et al. "A robust method to store complement C3 with superior ability to maintain the native structure and function of the protein." Frontiers in Immunology 13 (2022): 891994. Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only.
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