Creative Biolabs is a contract research organization with over 10 years of expertise in specialized areas. We combine expertise, world-class facilities, and cutting-edge instruments in all aspects of complement research to support companies involved in product development. Our experience in the field of complement services also enables us to work with you to develop experimental design and analysis of testing services to get the most out of your complement products.
C5 Functional Assay Protocol
C5 can be measured in a functional assay using C5-deficient mouse serum as a source of other C components. C5-deficient mice are readily available and the serum can be obtained commercially.
Fig.1 Flow chart of C5 functional assay protocol. (Creative Biolabs)
Published Data
In early atherosclerosis, underlying mechanisms remain incompletely defined, and reliable plasma biomarkers are lacking. In this study, deep quantitative proteomics of human aortic tissues, including healthy segments and regions exhibiting fatty streaks or fibro-lipidic plaques, revealed marked alterations. Proteins involved in lipid transport, complement activation, immunoglobulin functions, and hemostasis were upregulated, especially within the intima of fibro-lipidic lesions. Complement component C5 was confirmed by immunoblot, ELISA, and immunohistochemistry and linked to local activation. Elevated plasma C5 correlated with plaque burden and coronary calcification in two independent cohorts, highlighting its promise as a novel biomarker for subclinical atherosclerosis. Suggesting diagnostic promise.
Services and Product at Creative Biolabs
The complement team at Creative Biolabs will support you in all aspects of complement research. From study design and sample preparation to data analysis, integration, and interpretation, our contract research services are designed with scalability, quality, and repeatability in mind. From suggesting the best way to conduct research, to proactively updating the status of your project regularly, our services are exactly what you need. If you are looking for testing services or products related to complement research, please feel free to contact us for timely assistance.
Please note that our protocols are only for your reference!
For Research Use Only.
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