Creative Biolabs is a leading international CRO laboratory providing unmatched testing services to the pharmaceutical and biological research industries. We offer a variety of reliable complement analysis methods for the characterization of complement, leveraging the latest developments in the field of biotechnology and analytical science to provide our customers with unique complement testing solutions.
C5 Purification Protocol
Fig.1 Flow chart of C5 purification protocol. (Creative Biolabs)
Published Data
In a series of purification steps encompassing euglobulin precipitation, DEAE ion-exchange chromatography, and gel filtration, human complement proteins C5 and C3 were isolated with yields of approximately 41% and 20%, respectively. Employing Phenyl–Sepharose chromatography enabled complete resolution, as C3 was associated weakly with the resin while C5 adhered tenaciously, requiring a 50% glycerin solution for elution. Subsequent size exclusion chromatography efficiently eliminated co-purified contaminants, notably C4bp and H, from C5 fractions. The homogeneity of both proteins was validated by SDS–PAGE and immunochemical assessments, revealing that each comprises two disulfide-bridged polypeptide chains. This refined protocol underscores methodology precision and reliability.
Services and Product at Creative Biolabs
Creative Biolabs is committed to providing the highest quality service, timely and accurate results, and expert advice from highly qualified staff when needed. The reliability and accuracy of its data help customers make appropriate decisions about products. Our goal is to become the first choice for CRO companies in the US biological sector. If you are interested in our complement testing services and products, please contact our experts for more details, or visit our website to learn more about our range of services.
Please note that our protocols are only for your reference!
For Research Use Only.
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