In the previous report, it has shown that C6-deficient rabbit or rat serum can be used in C6 functional assay. What's more, the C6-depleted serum can also be obtained by passage of NHS using an anti-C6 immunoaffinity column. As an expert in the field of human complement system research, Creative Biolabs describes the C6 functional assay protocol for our clients all over the world.
C6 Functional Assay
Fig.1 Flow chart of C6 functional assay protocol. (Creative Biolabs)
Published Data
Researchers investigated the roles of complement effectors in collagen antibody-induced arthritis (CAIA) in mice, focusing on C3a and C5a receptors alongside the membrane attack complex. Histopathological and immunohistochemical analyses revealed that clinical severity was reduced by 52% in C3aR–/–, 94% in C5aR–/–, and 65% in C6-deficient mice relative to wild types, with corresponding decreases in tissue injury and IgG/C3 deposition. Additionally, synovial neutrophil counts fell across genotypes, while macrophage numbers declined in C3aR–/– and C5aR–/– mice. Synovial cytokine mRNA (TNF-α and IL-1β) levels were also diminished, suggesting a feedback loop that amplifies joint complement activation and inflammation.
With years of experience, our scientists at Creative Biolabs have developed a series of products and platforms related to human complement system research. We are therefore confident in offering the best and most suitable service for our customers all over the world. For customers' special project needs, our service can be tailor-designed to meet your specific needs.
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