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Functional assays of C7 are crucial for the study of the human complement system. Using an anti-C7 immunoaffinity column, the C7-deficient serum or C7-depleted serum can be obtained for C7 functional assay. Here, we describe the C7 functional assay protocol to promote your research.
C7 Functional Assay Protocol
Fig.1 Flow chart of C7 functional assay protocol. (Creative Biolabs)
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Published Data
Understanding the function of complement component C7 reveals its essential part in forming the membrane attack complex (MAC) and controlling immune responses. Current methods utilize high-sensitivity assays to measure C7 activity by analyzing purified proteins in physiological environments. These protocols consist of forming C5b-7 complexes on membranes before adding C8/C9 to evaluate MAC-induced cell lysis. Real-time monitoring of lytic activity becomes possible through analytical techniques like flow cytometry and fluorometric readouts, and the specificity of these methods is validated by testing with C7-deficient serum controls. The methods used are specifically designed to reduce non-specific interactions, resulting in consistent results throughout all experimental replicates.
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