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Encoded by the C7 gene in humans, complement component 7 (C7) is a component of the complement system involved in the innate immune system. As a part of the membrane attack complex (MAC), C7 can cause cell lysis and death by forming a hole in the surface of pathogens. For an in-depth study of C7, Creative Biolabs has developed the C7 purification protocol for our clients all over the world.
C7 Purification
Fig.1 Flow chart of C7 purification protocol. (Creative Biolabs)
Based on the established experimental method, we have been able to proficiently purify C7 to carry out the next work. We can also provide a series of products and services to meet the customer’s project and assay-specific requirements.
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Published Data
Fig.2 Identification of a C7 variant and analysis of amino acid residues within the visualized sequence alteration.1
The purification process for C7 must be meticulously controlled to ensure the protein maintains its structural and functional integrity. The latest purification methods use a series of chromatography steps beginning with affinity-based capture and followed by ion-exchange refinement to separate C7 from plasma-derived or recombinant solutions. The primary technical issues include reducing proteolytic breakdown while maintaining glycosylation structures, which are vital for binding performance. SEC-MALS and SDS-PAGE analytical techniques demonstrate monodispersity and glycan features, while functional assays prove complement-dependent lytic function. The use of low-temperature processing along with protease inhibitors greatly improves yield, which becomes especially important during therapeutic application scaling.
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Reference
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Massri, Mariam, et al. "Complement C7 and clusterin form a complex in circulation." Frontiers in Immunology 15 (2024): 1330095. Distributed under an Open Access license CC BY 4.0, without modification.
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