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Creative Biolabs is the world's leading CRO partner for the biopharmaceutical industries. From research to product commercialization, we provide the most advanced, complete analytical solutions for biotherapy. Complement factor B is a protein that in humans is encoded by the CFB gene. It is purified from normal human serum. Below we provide a CFB purification reference process so that you can quickly develop your research protocol.
CFB Purification Protocol
Factor B is a single-chain glycoprotein with a molecular weight of about 93K. It circulates as an inactive proenzyme (i.e., a zymogen), and only becomes activated after cleavage by the protein factor D.
Fig.1 Flow chart of CFB purification protocol. (Creative Biolabs)
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Published Data
The latest progressions in CFB purification focus on precise methods that enable large-scale production to satisfy strict research and therapeutic requirements. The streamlined protocol starts with mammalian system recombinant expression followed by multi-step chromatography to yield high-purity CFB. Affinity tagging enables selective binding, while ion-exchange chromatography removes contaminants before size-exclusion chromatography checks monomeric integrity. Validation after purification uses SDS-PAGE alongside mass spectrometry and functional assays to confirm glycosylation patterns, together with bioactivity. Deglycosylation research demonstrates that proteins maintain their ligand-binding abilities even after glycan removal, which highlights their structural robustness. These protocols provide >95% purity, which is crucial for both mechanistic investigations and preclinical research.
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Factor B is the central protease of the complement system of immune defense. It is part of the alternate pathway of the complement system and is cleaved by factor D into 2 fragments: Ba and Bb. Creative Biolabs combines an advanced technical analysis platform with a team of experts, making it a leading provider of analytical services for biopharmaceutical research. If you are working on complement or CFB research and hope our CFB purification protocol can help you, or you need CFB related detection and analysis service and related products, please do not hesitate to contact us, our team will have detailed communication with you, understand your needs and come up with a personalized solution.
For Research Use Only.
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