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Creative Biolabs provides cost-effective complement research solutions with a team of experienced scientists dedicated to providing first-class service to researchers. With more than a decade of experience, our talented and skilled staff is dedicated to getting the results you need. Factor B is a component of the alternative pathway of complement. It is a 64 kDa glycoprotein identified as a serine protease proenzyme whose active form activates the proc lotting enzyme to clotting enzyme. The following is a general summary of the CFB functional assay protocol, hoping to be helpful to your research.
CFB Functional Assay Protocol
Fig.1 Flow chart of CFB functional assay protocol. (Creative Biolabs)
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Published Data
CFB plays a pivotal role in the alternative pathway of the complement system, where its proteolytic activation drives the formation of C3 convertase. Functional assays to quantify CFB activity often rely on measuring its cleavage into Ba and Bb fragments under controlled conditions. Recent studies highlight methodologies combining recombinant CFB with stabilizing buffers to preserve enzymatic activity, followed by kinetic analysis using fluorescence-based substrates or gel electrophoresis to monitor fragment generation. Precise quantification of CFB-C3b interactions via surface plasmon resonance (SPR) further validates binding affinities, while inhibition assays using monoclonal antibodies demonstrate therapeutic targeting potential. These approaches ensure reproducible, high-throughput data critical for drug discovery and diagnostic development.
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As the global leader in R&D genome services, Creative Biolabs is leading the way in providing superior data quality and unparalleled technical support, enabling researchers around the world to advance their scientific discoveries faster than ever before. If you are interested in our complement testing services or products, please do not hesitate to contact us, even if it is just to communicate CFB functional assay protocol.
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