CFD Purification Protocol

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CFD Purification Protocol

Factor D is a 24 kDa serine protease comprising 228 amino acids, also known as adipsin. Unlike most proteins of the complement system, which are synthesized by the liver and immune cells, factor D is predominantly produced by and secreted into the bloodstream by adipocytes. Plasma concentration of FD is very low and alternative sources have often been used, notably, urine from patients with renal tubular dysfunction or peritoneal dialysis fluid. The following method has been developed to purify factor D from plasma.

Flow chart of CFD purification protocol. Fig.1 Flow chart of CFD purification protocol. (Creative Biolabs)

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