Complement C5b-9 Binding to Cells Quantification Protocol

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Complement C5b-9 Binding to Cells Quantification Protocol

Activation of complement is very common in many diseases and is closely linked to the pathogenesis of these diseases. There is evidence revealed that the deposition of C5b-9 complexes at toxic doses resulted in plasma membrane perforation and necrotic cell death. In addition, C5b-9 binding to cells may be related to apoptosis. Here, we describe a flow cytometry method to quantify the deposition of C5b-9 on cells in vitro.

Flow chart of C5b-9 binding to cells quantification protocol. Fig.1 Flow chart of C5b-9 binding to cells quantification protocol. (Creative Biolabs)

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