Target Genome Editing

Genome editing, also known as genetic engineering or genetic modification, is a genetic tool that directly manipulates the gene makeup of an organism by inserting, deleting or replacing genome at a specific site using biotechnology.

Creative Biolabs uses a powerful and innovative tool called the CRISPR system to knock out or silence genes, suppress gene expression or unregulated genes to increase protein expression. This advanced technology used by our professionals can easily modify the gene expressions, screen potential targets and study how changes on genome may eventually affect organisms.

The types of our genetic manipulation techniques include but are not limited to:

Gene Knockout

Platforms Nuclease Recruiter Advantages
CRISPR/Cas9 System RNA
  • Small guide RNA serves as the recruiter
  • Simplified design and construction
  • High efficiency
  • High Throughput Screening
  • Potentially to cleave every gene
Transcription Activator-like Effector Nuclease Protein
  • Programmable with modular arrays
  • Each module recognizes a single base pair

Gene Overexpression

We provide gene overexpression to help our clients in various scientific researches as follows:

  • Drug target identification and validation which offers insight into the function of target proteins.
  • Overexpress wild-type protein in the mutant background to further study the drug-related pathway.
  • Use the overexpression phenotype in epistasis tests.
  • Overexpress the mutant protein to revert the deficiency.
  • Generate mutations when loss-of-function experiment is not available.
  • Use output to create interaction networks.
  • Use overexpression phenotype as a starting point for modifier screens.
  • Overexpress in heterologous organism.

Gene Conditional Overexpression

Our creative functional genomics approach tailored for diploid organisms allows conditional knockout of genes. This innovative approach is used by combining target gene replacement of one allele with conditional gene expression of another. Our platform demonstrates the reproducibility and reliability of determining the essentiality of genes, and is well suited for systems and large-scale essential gene identification and validation of diploid pathogenic microorganisms.

Gene Silencing

Our platform of RNAi services design allows high-level expression of shRNA and miRNA that will help our clients in various scientific research as follows:

  • siRNA or shRNA design and synthesis service
  • siRNA or shRNA vector construction service
  • siRNA or shRNA transfection service
  • Test mRNA expression by PT-PCR and the protein expression by Western Blot

For more details, please feel free to contact us or send us a query directly.

For Research Use Only | Not For Clinical Use

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