C4b-binding protein (C4BP) acts as a critical inhibitor of the classical as well as lectin pathways by limiting the effects of activated C4b. It is worth noting that C4BP plays a critical role in limiting the generation of the C3 convertase C4b2a. C4BP is closely linked to the natural decomposition of C3 convertase. These functions allow C4BP to serve as the primary target for pathogens to evade complement attacks. With our extensive experience and industry-leading knowledge, Creative Biolabs has developed an unmatched platform for complement identification and functional characterization.
C4BP Purification Protocol
C4BP is a soluble 570 kDa polyplasmic glycoprotein expressed primarily in the liver. It is composed of several identical α-chains and a β-chain, which are mainly composed of the specific domain. The concentration of C4BP in plasma is about 200 mg/L. Here, we describe a simple protocol to purify C4BP from human plasma.
Fig.1 Flow chart of C4BP purification protocol. (Creative Biolabs)
With many years of experience and an advanced analytical platform, Creative Biolabs has gradually become a leading company in the development of complement related products meeting strict quality standards. Our diverse complement products such as sera, proteins, kits and aptamers have been proven trustworthy in multiple fields. We firmly believe that the future of complement research is bright. Furthermore, as an end-to-end company providing high-quality services, our diverse complement assay and development services give us a significant advantage in assisting clients around the world.
Services and Product at Creative Biolabs
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Please note that our protocols are only for your reference.
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