Complement C9 has been studied for its pivotal role in forming the channel part of the membrane attack complex (MAC). Monomeric C9 (mC9) binds the C5b-8 complex to form C5b-9 which catalyzes the polymerization of C9 forming C5b-p9 and transmembrane channels leading to lysis of the target cell. Patients with C9 deficiency suffer from recurrent bacterial infections. Creative Biolabs is a provider of outsourced professional laboratory testing services for drug discovery, catering to small pharmaceutical companies and biotechnology companies. Here we summarize the C9 functional assay protocol for your reference.
C9 Functional Assay Protocol
C9 can be measured in a functional assay using C9-depleted serum obtained by passage over an anti-C9 immunoaffinity column.
Fig.1 Flow chart of C9 functional assay protocol. (Creative Biolabs)
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