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As the global leader in CRO services, Creative Biolabs is leading the way in providing unmatched technical support and superior data quality, enabling researchers around the world to advance their scientific discoveries at unprecedented speeds. Our price is the most competitive in the industry. You can always get a better price from us without sacrificing quality and service.
C9 Purification Protocol
Complement component 9 (C9) is a MACPF protein involved in the complement system, which is part of the innate immune system. C9, a single-chain plasma protein (molecular weight 69 kDa), binds the α-chain of C8 in the C5b-8 complex and undergoes a major conformational change from a globular, hydrophilic form to an elongated, amphipathic form which traverses the membrane and exacerbates membrane leakiness.
Fig.1 Flow chart of C9 purification protocol. (Creative Biolabs)
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Published Data
Efficient purification of native C9 requires sequential chromatographic strategies. Initial cation-exchange chromatography effectively separates C9 from plasma-derived complement components, leveraging pH-dependent elution gradients. Subsequent affinity-based steps enhance specificity, while size-exclusion chromatography (SEC) confirms monodispersity. Notably, maintaining physiological salt concentrations preserves structural integrity, and protease inhibitors prevent degradation during processing. SDS-PAGE and Western blotting validate purity (>95%), with mass spectrometry confirming the absence of truncated isoforms. Such protocols align with the glycosylation analysis seen in C2 purification, where deglycosylation and SEC-MALS ensure homogeneity, a principle critical for functional studies.
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Creative Biolabs is a bio-CRO company with a long-term commitment to research and development of related technologies in the field of biomedical research. We have been assisting research institutes and pharmaceutical companies to design project plans. Through continuous efforts to improve expertise and platform building to help you achieve your research goals in the complement field, you can quickly drive your results. Please feel free to contact us to further communicate your research needs and we are happy to assist you.
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