CD59 Functional Assay Protocol

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CD59 Functional Assay Protocol

This assay is based on the ability of CD59 to reincorporate into a cell through its GPI anchor. Guinea pig erythrocytes (GPE) are the ideal target cells. Chicken E is an acceptable alternative but sheep E, pig E, or rabbit E are unsuitable, due to the high level of endogenous CD59 in these cells.

Flow chart of CD59 functional assay protocol. Fig.1 Flow chart of CD59 functional assay protocol. (Creative Biolabs)

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