Creative Biolabs is always at the forefront of biotechnology and committed to providing our clients with high-quality, efficient and flexible services to promote your complement-related experimental project. As a receptor for specific cleavage fragments of complement component 3, complement receptor 2 (CR2, CD21) participates in the transport of immune complexes and retention in immune follicles, lowers the stimulation threshold of B cells, and acts as an essential part of complement-driven immune regulation in humoral immunity. CR2 is only expressed in mouse/human B cells and follicular dendritic cells, so the detection of CR2 can be used to identify and characterize B cell populations.
Here, we briefly describe the protocol for detecting CR2 on mouse splenic B cell subsets. It is worth noting that this protocol can also be extended for the detection of CR2 in cells from other sources (peripheral blood, bone marrow, peritoneal cavity, etc.) with simple modifications. A similar procedure can also identify cells with antibodies that recognize human epitopes.
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CR2 on Mouse Splenic B Cells Detection Protocol
Fig.1 Flow chart of CR2 on mouse splenic B cells detection. (Creative Biolabs)
Creative Biolabs is confident to meet the various needs of scientific researchers and provides them with high-quality and efficient services. You may purchase or customize various reagents that may be needed in experiments conveniently, and we are also capable of providing you with real-time technical support and assistance to promote your entrusted detection assays or other complement-related procedures to provide reliable and repeatable data support for your projects.
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