Creative Biolabs provides contract services for complement research. The broad base of experience of our employees, as well as our ability to assemble multidisciplinary project teams, has repeatedly proved to be invaluable assets in carrying out new R&D projects. Here we briefly summarize the esterolytic assay Protocol using different substrates.
Esterolytic Assay Protocol
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ZLNE Substrate
Functional activities of C1r and C1s can be measured using the chromogenic substrate ZLNE.
Fig.1 Flow chart of esterolytic activity analysis of C1r and C1s protocol by using substrate ZLNE. (Creative Biolabs)
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AGLMe Substrate
The following assay uses the AGLMe substrate and is based on indirect measurement of methanol released upon cleavage of the methyl ester.
Fig.2 Flow chart of esterolytic activity analysis of C1r and C1s protocol by using substrate AGLMe. (Creative Biolabs)
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BAEe Substrate
The following assay uses the BAEe substrate and is based on indirect measurement of ethanol released upon cleavage of the ethylester. This assay is less sensitive than the AGLMe assay but is specific for C1s since BAEe is not cleaved by C1r.
Fig.3 Flow chart of esterolytic activity analysis of C1r and C1s protocol by using substrate BAEe. (Creative Biolabs)
Complement-related Services and Products
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Complement Products
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Complement Test Services
At Creative Biolabs, we are committed to providing best-in-class testing services and high-quality products for our customers' complement testing needs. We are passionate about providing high-quality service, fast turnaround times, and excellent customer support. If you are interested in our complement services or products, please do not hesitate to contact us for more details.
Please note that our protocols are only for your reference!
For Research Use Only.
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