Creative Biolabs is a growing team of experts with multidisciplinary backgrounds dedicated to implementing and optimizing robust complement detection services and developing products to accelerate complement research. By engaging and working closely with end users to develop comprehensive services, we have built an easy-to-use and powerful analytics platform to meet current and future research needs. Here, we simply summarized C1r and C1s functional analysis protocol to provide a reference for customer research.
Functional Characterization of C1r and C1s
Fig.1 Flow chart of C1r and C1s functional analysis protocol. (Creative Biolabs)
Published Data
In the classical complement pathway, C1r, a serine protease, triggers activation, playing a vital role in innate immunity against pathogens and modified cells. C1r self-activates and subsequently cleaves C1s, important for this activation process. Researchers opted to examine a CCP1-CCP2-SP variant of zymogen C1s for cleavage by recombinant and plasma-purified C1r. For this, recombinant zymogen C1s was reacted with recombinant C1r, analyzed via SDS-PAGE, and detected using antibodies targeting the SP domain of C1s and polyclonal C1r antibodies. The data displayed the substrate consistently cleaved by C1r, enhancing the assessment of C1r's specificity.
Fig.2 SDS-PAGE results show the activation and cleavage of zymogen C1s by serine protease C1r.1
Services and Products
-
Complement Products
-
Complement Test Services
Creative Biolabs is proud of the expertise we have. Since our inception, we have had a strong capability in complement analysis, combined with its application in biotechnology and pharmaceutical research. We are very proud of what we have achieved over the past decade. As a complement solutions provider, we are grateful that we are still serving many of our customers from more than a decade ago. It confirms that we are doing the right thing. We attach great importance to cooperation with universities, research institutions, and the R&D community. If you are interested in our complement services or products, please do not hesitate to contact us.
Please note that our protocols are only for your reference!
Reference
-
Wijeyewickrema, Lakshmi C., et al. "Molecular determinants of the substrate specificity of the complement-initiating protease, C1r." Journal of Biological Chemistry 288.22 (2013): 15571-15580. Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only.
Related Sections:
