Creative Biolabs has been a long-term expert in the field of complement therapy. Based on our extensive experience and advanced platforms, we are confident in offering a full range of complement therapeutic products and services for our clients all over the world. For example, recombinant full-length C1r and C1s can be easily expressed using our established insect expression system. Here, we describe the recombinant C1s production and purification protocol to promote your research.
Recombinant C1s Production and Purification Protocol
Fig.1 Flow chart of recombinant C1s production and purification. (Creative Biolabs)
Published Data
The cDNA of hamster complement C1s was subcloned into the expression vector BCMGSNeo and introduced into SEA7 cells, a polyoma virus-transformed A31 mouse fibroblast line. The transfected cells secreted significant amounts of recombinant C1s, which became activated in a serum-free medium and exhibited hydrolytic activity on acetyl-Gly-l-Lys-naphthyl ester (AGLNE). Purification of C1s to homogeneity from the culture medium involved size-exclusion HPLC and other methods. The purified hamster C1s was capable of depleting human complement in hemolytic assays and hydrolyzing gelatin as demonstrated in enzymographic studies.
As a forward-looking research as well as a leading custom service provider in the field of complement therapy, Creative Biolabs has won a good reputation among our worldwide customers for accomplishing numerous challenging projects. Our service portfolio includes therapeutic antibodies, serine protease inhibitors, soluble complement regulators, complement component inhibitors, as well as anaphylatoxin receptor antagonists, and other small molecules.
To learn more details, you can get access with the following links:
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Complement Products
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Complement Test Services
If you are interested in our products and services, please do not hesitate to contact us for more information.
Please note that our protocols are only for your reference!
For Research Use Only.
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