C1r, C1s, and the C1s-C1r-C1r-C1s Tetramer Biochemical Analysis Protocol

Creative Biolabs is a leading provider of services and products in the field of complement research. In our state-of-the-art laboratory, we have optimized many test methods. We support our customers' needs with easy-to-interpret reports, high-quality products, and fast delivery of results.

Biochemical Characterization of C1r, C1s, and the C1s-C1r-C1r-C1s Tetramer

SDS-PAGE analysis is used at different purification stages to identify the C1r- and/or C1s-containing fractions and to quantify the C1r and C1s protein contents of insect cell culture supernatants before reconstitution of the recombinant tetramer. Performing this analysis under reducing conditions allows discrimination between the single-chain proenzymes and the two-chain activated proteases.

Flow chart of C1r, C1s, and the C1s-C1r-C1r-C1s tetramer biochemical analysis protocol.

Fig.1 Flow chart of C1r, C1s, and the C1s-C1r-C1r-C1s tetramer biochemical analysis protocol.

Complement-related Services and Products at Creative Biolabs

  1. Complement Products
  2. Complement Test Services

Creative Biolabs has worked with a large number of clients for over a decade. Our business is sustained by our talented scientists working creatively, and diligently to deliver results and achieve project goals. This is reflected in our past success with many R&D projects. To further illustrate our capabilities and experience in the complement field, you can contact our experts at any time to discuss your project and requirements.

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