Variable Region Sequencing and Cloning

As a critical step of antibody molecular biology services, antibody variable region (VH and VL) sequencing and cloning are very important for antibody construction. Creative Biolabs is committed to providing a one-stop solution for superior therapeutic antibody discovery and development, and we offer this key first step-variable region sequencing and cloning service.

Antibody Variable Region Sequencing and Cloning Services

Antibody variable region sequencing from hybridomas or clonal B cells is a critical first step in a broad range of applications, such as the production of recombinant antibodies in different formats, antibody humanization and affinity maturation, antibody engineering, etc. At Creative Biolabs, we offer variable region sequencing services including heavy and light chains from both hybridoma cell lines and clonal primary B cells derived from a wide range of species. Following the antibody variable region sequencing service, the variable regions can be reformatted into various types and transiently be produced in non-mammalian or mammalian cells. Once sequenced, we offer cloning service to deliver both sequence results and clone constructs to our clients.

We offer the following service types (including but not limited to). Please contact us for further communications about your specific project.

• Sequencing and cloning of mouse hybridoma IgG variable regions;
• Sequencing and cloning of rat hybridoma IgG variable regions;
• Sequencing and cloning of rabbit hybridoma IgG variable regions;
• Sequencing and cloning of human hybridoma IgG variable regions;
• Sequencing and cloning of primate B cell IgG variable regions;
• Sequencing and cloning of hamster hybridoma IgG variable regions;
• Sequencing and cloning of mouse hybridoma IgM variable regions;
• Sequencing and cloning of rat hybridoma IgM variable regions;
• Sequencing and cloning of primate B cell IgM variable regions.

Fig.1 Our one-stop solution flow chart.

Once sequenced, we will provide study report containing analyzed sequence results to you. Once cloned, the final plasmid will be shipped to you. If amplified PCR fragments are needed, please let us know, and we will proceed accordingly.

To meet your desired objectives, Creative Biolabs is dedicated to offering the high quality and efficient sequencing and cloning services to best tailor your projects. In addition, we also provide a full spectrum of functional assays to evaluate the antibody characteristics and functions. To get more information, please feel free to contact us.