Mannose-binding lectins (MBLs) are oligomeric proteins of the innate immune system. As a lectin, it plays a crucial role in innate immunity. Natural MBL is a heterogeneous composition of oligomers of several structural units. Recombinant synthesis in mammalian cell lines often results in the production of compositions of small oligomers, the oligomeric structure of MBL consisting of subunits comprising three similar peptide chains, each approximately 30 kDa. Although MBL can form several oligomeric forms, there are data suggesting that dimers and trimers are biologically inactive as opsonins and that at least the tetrameric form is required for complement activation. Creative Biolabs is committed to the development of the future of complement science, contributing leading analytical technologies and extensive experience for customized solutions.
Affinity Chromatography-Based Fractionation of MBL Oligomers Protocol
Studies have revealed that the oligomeric state of MBL is closely related to the binding properties and effector functions of MBL. Here, we describe a simple procedure for the fractionation of MBL oligomers.
Fig.1 Flow chart of fractionation of MBL oligomers. (Creative Biolabs)
As an expert in the field of complement, Creative Biolabs focuses on providing a wide range of complement products and complement detection services according to the individual needs of customers. Various complement products, such as peptides, proteins, serum and aptamers, can meet your different research needs. Committed to being your best complement research partner, we provide our customers with comprehensive complement detection services to help your research on complement applications and functions. In addition to our diverse services, we also welcome special requests from customers.
Services and Product at Creative Biolabs
If you have any questions about complement research, please do not hesitate to contact us to get a reasonable solution.
Please note that our protocols are only for your reference.
For Research Use Only.
Related Sections:
