Mannose-binding lectin (MBL) is a serine protease that plays a crucial role in innate immunity as an opsonin. With unique recognition capabilities, MBL can bind to the surface of a variety of pathogenic microorganisms, including bacteria, viruses, protozoa and fungi, leading to the activation of the lectin pathway of the complement system. Another crucial function of MBL is that the molecule binds to senescent and apoptotic cells and enhances the phagocytosis of apoptotic cells and cellular debris by phagocytic cells. After decades of expansion and development, Creative Biolabs has become an expert in the field of complement. Here we share a simple procedure for MBL purification.
MBL Purification Protocol
The purification of MBL depends on carbohydrate affinity chromatography and the sequential use of different sugars to remove contaminating anti-carbohydrate antibodies from MBL preparations.
Fig.1 Flow chart of MBL purification protocol. (Creative Biolabs)
With years of experience in complement research, Creative Biolabs is committed to providing comprehensive services and diversified products for complement research. Creative Biolabs is proud to offer a full range of complement-associated antibody products, allowing the detection of complement-associated by various techniques such as WB and ELISA. We also offer a range of other complement products such as kits, inhibitors, sera, aptamers and peptides for complement identification and characterization. In addition, we provide custom antibody development services, such as complement function and activity testing, to meet the specific needs of our customers.
Services and Product at Creative Biolabs
If you have any difficulties with complement research, please contact us promptly to get a reasonable solution.
Please note that our protocols are only for your reference.
For Research Use Only.
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