Mannan-binding lectin (MBL) refers to an oligomeric protein in the innate immune system. In recent years, two procedures for the fractionation of MBL oligomers have been established. Classical affinity chromatography can be applied to enrich the abundance of large MBL oligomers, while large-volume ion exchange chromatography has been used for the separation of MBL oligomers into fractions with an enhanced size homogeneity. Here, Creative Biolabs describes the novel ion exchange chromatography-based separation of MBL oligomers protocol to promote your research.
Fig.1 Flow chart of ion exchange chromatography-based separation of MBL oligomers protocol. (Creative Biolabs)
Creative Biolabs is well equipped and versed in the field of the human complement system to assist our customers’ research and project development. We can not only provide super high-quality data but also help you establish a custom research pipeline and create new techniques for your special projects.
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Please note that our protocols are only for your reference.