Creative Biolabs is a biological expert specializing in cell biology and immunology research. Thanks to our strong independent development and research capabilities, we provide scientists around the world with the most comprehensive and convenient one-stop complement-related research solutions.
The activation of the complement-activated mannan-binding lectin (MBL) pathway is similar to the classical pathway (CP). MBL recognizes and interacts with the mannose residues on the pathogenic microorganisms and undergoes conformational changes, then activates downstream serine proteases with similar biological activities and functions to C1s, MASP-1 and MASP-2. MASP-2 hydrolyzes C4 and C2 molecules, while MASP-1 directly cut C3, and then forms C3 convertase. The MBL pathway exerts complement functions by activating the same membrane attack unit as the CP. The lectin pathway provides another antibody-independent way to stimulate the complement system. Here, we briefly describe the experimental procedures for detecting the complement function of the MBL pathway in samples to provide technical references for your experimental project.
MBL Pathway of Complement Analysis Protocol
The novel method to verify the function of the MBL pathway is carried out by ELISA theory.
Fig 1. Workflow of MBL pathway of complement analysis protocol.
Time-Resolved Immunofluorescence Assay For Mbl Function Test Protocol.
Time-resolved immunofluorescence assays were also used to determine the ability of serum or plasma samples to mediate activation of complement factor C4 via the MBL pathway of complement.
Fig 2. Workflow of time-resolved immunofluorescence assay protocol. (Creative Biolabs)
Excellent research results are inseparable from mature and comprehensive data support and experimental verification, and Creative Biolabs is always committed to providing high-quality biology-related experimental services for researchers. We have deeply studied biotechnology for many years with cutting-edge patented technology and engineering teams, and have established stable cooperative relationships with many scientific research institutions around the world. You may directly purchase our mature and stable high-quality experimental devices and reagents to carry out research by yourself. We are also willing to complete any of your commissions and provide technical support so that you have the opportunity to advance your research plan rapidly and favorably. With our strong support and assistance, we provide you the chance to fully explore the infinite possibilities of biology and immunology.
Services and Products at Creative Biolabs
Please do not hesitate to contact us with your need or for more information.
For Research Use Only.
Related Sections:
