Chromatography-Based C1q Purification Protocol

Creative Biolabs has spent many years of effort in the complement research field. We have built a comprehensive complement-related assay platform. Here, we briefly summarize the chromatography-based C1q purification protocol for our customers, which includes two methods, chromatography on the ion-exchange column and size exclusion column, as well as chromatography on the IgG-Sepharose affinity column and FPLC on the strong cation exchange column.

1. Purification of C1q by Ion-exchange Column and Size Exclusion Column

Fig.1 Flow chart of C1q purification by ion-exchange column and size exclusion column. (Creative Biolabs)

1. Purification of C1q by IgG-Sepharose Affinity Column and FPLC on Strong Cation Exchange Column

Fig.2 Flow chart of C1q purification by IgG-Sepharose affinity column and FPLC on strong cation exchange column. (Creative Biolabs)

Creative Biolabs offers a range of complementary products such as native complement protein, human and animal sera, complement inhibitors, complement assay reagents, as well as ELISA Kits. Complement assay services are also available in our lab, including complement function/activity test, autoantibody test, genetic test...

To learn more details, you can get access with the following links:

1. Complement Products
2. Complement Test Services

For more information, please feel free to contact us.

• Collagen-Like Region (CLR) of C1q Isolation Protocol
• gC1q Fragment Isolation Protocol
• C1q Fragments Expression and Purification Protocol
• C1q Biochemical Analysis Protocol
• C1q Function Analysis Protocol
• C1q Hemolytic Assay Protocol
• C1q-Based Inhibition of Hemolysis Protocol
• Interaction of C1q with Human Aggregated IgG Detection Protocol