Reporter-encoding Oncolytic Vaccinia Virus Western Reserve (ΔE3L,ΔK3), pSE-(luciferase) (RepOV-0077WQ)

This product is a luciferase encoding oncolytic vaccinia virus, which is based on VACV-WR with E3L and K3L double deleted.Protein E3 plays a role in the inhibition of multiple cellular antiviral responses activated by dsRNA, such as inhibition of PKR activation, apoptosis, and IFN-mediated antiviral activities. Protein K3 acts as a pseudosubstrate for EIF2AK2/PKR kinase. Inhibits therefore eIF-2-alpha phosphorylation by host EIF2AK2/PKR kinase and prevents protein synthesis shutoff.The double deletion of E3L and K3L with oncolytic-rendered modifications could enhance an immune response to a poxvirus vaccine.This product can be used in oncolytic virotherapy research and vaccinie application.

Specifications
Family Poxviridae
Species Vaccinia virus
Serotype Western Reserve
Backbone VACV-WR(ΔE3L,ΔK3)
Backbone Background VACV-WR strain derived from Wyeth through passaging in mice and shown high tumor selectivity and strong oncolytic effect in mouse models.The engineered VACV-WR could further enhance the immune activity and the efficacy of cancer therapies.
Gene Modification ΔE3L,ΔK3
Promoter pSE
Transgene luciferase
Type of Transgene Reporter gene
Related Target/Protein Luciferase
Capsid Modification None
Titer >1*10^8 PFU
Related Diseases Breast cancer, Prostate cancer, Colon cancer, Cancer vaccine
Transgene
Alternative Names luciferase, Fluc
Information
Introduction Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein. Luciferases are widely used in biotechnology, for microscopy and as reporter genes, for many of the same applications as fluorescent proteins. However, unlike fluorescent proteins, luciferases do not require an external light source, but do require addition of luciferin, the consumable substrate.
All services and products are for lab research only, not for any clinical use.

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