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Anaphylatoxin C5a Functional Assay Protocol
Numerous assays for the activities of the anaphylatoxins have been used, including smooth muscle contraction, neutrophil chemotaxis assays, and neutrophil oxidase assays. We briefly summarize the neutrophil chemotaxis assay protocol.
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Neutrophil Chemotaxis Assays
Fig.1 Flow chart of Neutrophil Chemotaxis Assays. (Creative Biolabs)
Published Data
Fig.2 Immunofluorescence analysis reveals the binding of C5a to neuronal C5aR.1
Complement C5a has been identified as a key player in tissue regeneration, acting via its receptor C5aR, which is expressed in the nervous system—a region where therapeutic options for repair remain limited. Researchers investigated the influence of C5a on axonal regrowth using an in vitro axotomy paradigm. Primary hippocampal neurons from embryonic rats were isolated; RT-PCR confirmed C5aR mRNA, while immunofluorescence demonstrated membrane localization, NF-L co-localization in mature neurons, and receptor phosphorylation triggered by C5a. Administering C5a in a 3D-printed microfluidic device significantly accelerated both axonal growth speed and extent, suggesting a promising regenerative role with therapeutic potential in central nervous system injuries.
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Please note that our protocols are only for your reference!
Reference
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Cotten, Aurélie, et al. "Complement C5a Implication in Axonal Growth After Injury." Cells 13.20 (2024): 1729. Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only.
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