Creative Biolabs is a close-knit team of passionate scientists, engineers, and creative thinkers, all working together toward our common goal of a future where drugs can be discovered faster and delivered to patients more cost-effectively. We briefly summarize the anaphylatoxin C5a purification protocol to promote your research.
Anaphylatoxin C5a Purification Protocol
Fig.1 Flow chart of anaphylatoxin C5a purification protocol. (Creative Biolabs)
Published Data
In leukocytes, C3a and C5a trigger chemotaxis through a Gi-dependent mechanism that is susceptible to inhibition by pertussis toxin. Using a fluid-phase convertase system comprising cobra venom factor (CVF) and Bb, human C3a and C5a were derived from C3 and C5 and subsequently purified via DEAE chromatography with an imidazole HCl/NaCl buffer, followed by adsorption and NaCl elution. Remarkably, only C5a induced chemotactic migration in immortalized human dermal microvascular endothelial cells. This response was impeded by PI3-kinase, src kinase, and EGFR inhibitors, as well as neutralizing antibodies against EGFR and heparin-binding EGF-like factor, with C5a stimulation promoting EGFR phosphorylation.
Services and Product at Creative Biolabs
Creative Biolabs is an independent contract laboratory offering a full range of complementary testing and analytical services. We are considered an authority in the field of complement detection. Our complement testing laboratory consists of a team with decades of experience in complement research, who have already provided customized complement testing services and products to researchers in the pharmaceutical, biological, and other industries. Please do not hesitate to contact us, our team will provide you with more detailed service or product information.
Please note that our protocols are only for your reference!
For Research Use Only.
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