C5a-Mediated Chemotaxis of Bone Marrow-Derived Neutrophils Protocol

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C5a-mediated Chemotaxis of Bone Marrow-Derived Neutrophils Protocol

C5a is chemotactic and anaphylactic ally toxic. C5a is closely related to many biological effects, including increased vascular permeability, inflammatory cell chemotaxis as well as cytokine and chemokine release. In addition, C5a is also closely relevant to adaptive immunity. Studies have shown that myeloid-derived cells undergo chemotaxis, degranulation, and secretion after activation by C5a. Here we provide a simple protocol for the detection of C5a-mediated chemotaxis of bone marrow-derived neutrophils.

Flow chart of C5a-mediated chemotaxis of bone marrow-derived neutrophils protocol. Fig.1 Flow chart of C5a-mediated chemotaxis of bone marrow-derived neutrophils protocol. (Creative Biolabs)

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