One-Stop
ASO Delivery Services
Overcoming biological barriers through optimized conjugation and nanoparticle platforms. We provide high-efficiency antisense oligonucleotide solutions for gene therapy research.
Revolutionizing ASO Therapeutics
Antisense Oligonucleotides (ASOs) are powerful tools for gene silencing and splice modulation. However, their clinical potential is often limited by rapid enzymatic degradation and poor tissue-specific uptake.
"Our delivery platform bridges the gap between genomic precision and biological accessibility, ensuring ASOs escape endosomal entrapment and reach their intracellular targets."
Creative Biolabs offers integrated solutions to tackle critical ASO delivery challenges, including serum stability, membrane penetration, and precise tissue targeting across CNS, oncology, and metabolic disorders.
Biological Barriers to ASO Delivery
Enzymatic Decay
Naked ASOs are rapidly degraded by nucleases in the bloodstream before reaching target tissues.
Membrane Barriers
Negatively charged ASOs struggle to cross the hydrophobic lipid bilayer of cell membranes.
Endosomal Trap
Most ASOs enter cells via endocytosis but remain trapped in endosomes, leading to lysosomal degradation.
Off-target Accumulation
High accumulation in liver and kidney often causes toxicity while limiting delivery to CNS or heart.
Comprehensive ASO Delivery Solutions
Leveraging dual expertise in nucleic acid chemistry and advanced formulation, we provide end-to-end services to transform therapeutic concepts into validated drug candidates.
Custom Conjugation
Design and synthesis of high-purity ASO conjugates tailored to specific ligands (GalNAc, Peptides) or antibodies (AOCs) to bypass biological barriers.
Formulation Engineering
Optimization of LNPs, exosomes, and polymeric carriers to ensure long-term stability in systemic circulation and maximized endosomal escape.
Advanced Bio-Validation
Rigorous PK/PD evaluation, tissue biodistribution studies, and functional gene knockdown assays to ensure therapeutic potency and safety.
ASO Delivery System
GalNAc Conjugates
GalNAc is a carbohydrate fragment bound to ASPGR. ASPGR is highly expressed in the liver, so it can deliver ASO drugs to the liver with high efficiency and specificity.
During endosomal acidification, GalNAc fragments are degraded by enzymes, resulting in the release of oligonucleotides.
Aptamer Conjugates
Aptamer conjugates represent a versatile tool in molecular therapy, as aptamers can be considered “chemical antibodies” that bind to their respective target proteins with high affinity.
Their advantages include simple manufacturing, low cost, small size, and low immunogenicity.
Peptide Conjugates
Peptides that can effectively target antisense oligonucleotides (ASOs) to specific tissues or cells play a crucial role in enhancing the therapeutic efficacy of gene silencing therapies.
CPPs (Cell-Penetrating Peptides) are short amphotic or cationic peptide fragments that can be chemically coupled directly to charge-neutral ASO to exert antibacterial and antiviral effects.
Lipid Nanoparticles (LNPs)
LNPs contain four basic components: cationic or ionized lipids, cholesterol, helper lipids, and polyethylene glycol (PEG) lipids.
After polyanionic nucleic acid drugs are mixed with lipids, nucleic acids aggregate to trigger endocytogenic uptake of nanoparticles suitable for delivery to organs/tissues with abundant capillary systems.
Exosome
Exosomes are lipid bilayer-coated vesicles with a diameter of about 100nm, capable of crossing biofilms, immune phagocytosis, long cycle, non-toxic.
Some also have pro-regeneration and anti-inflammatory properties that can enhance the effect of ASO delivery.
Spherical Nucleic Acid (SNA)
SNA particles are composed of hydrophobic core nanoparticles whose surface hydrophilic ASO is densely packed on the surface via mercaptan bonds.
It has the potential to cross the blood-brain barrier and is also used for local delivery to skin keratinocytes.
ASO Delivery Strategies
We offer a versatile toolkit of delivery technologies designed to optimize the stability, pharmacokinetics, and tissue-specific activity of your antisense oligonucleotides.
Conjugation-Based ASO Delivery
Direct Molecular Engineering
Chemical attachment of specific ligands such as GalNAc for hepatocyte targeting, specialized peptides for cellular entry, or small molecules to enhance tissue uptake.
Harnessing the high specificity of monoclonal antibodies (AOCs) to deliver ASO payloads to extra-hepatic tissues like muscle, heart, or specific tumor types.
Improving serum stability and membrane affinity through direct covalent coupling with hydrophobic lipids or biocompatible polymer chains.
Nanoparticle Delivery Systems
Advanced Encapsulation
The gold standard for nucleic acid delivery. Our optimized LNP formulations protect ASOs from nuclease degradation and ensure highly efficient endosomal escape.
Utilizing synthetic or natural polymers to create pH-responsive particles that release the ASO payload specifically within the intracellular environment.
Innovative combination of lipid and polymer components to balance particle stability with biocompatibility and low immunogenicity.
Intracellular Trafficking &
Endosomal Escape
Entering the cell is only half the battle. Our platform focuses on maximizing cytosolic and nuclear delivery by engineering systems that trigger efficient endosomal disruption, ensuring ASOs reach their intended molecular targets before degradation.
pH-Sensitive Linkers
Engineered to release payloads specifically within the acidic environment of endosomes.
Membrane Disruptors
Utilizing cell-penetrating peptides to enhance direct translocation and escape efficiency.
Custom Delivery Workflow
Strategy Design
Target definition and carrier selection based on tissue type.
Optimization
Formulation tuning and conjugation chemistry validation.
Validation
In vitro screening and intracellular localization assays.
Evaluation
In vivo PK/PD studies and efficacy assessment.
Why Partner with Creative Biolabs?
Customized Solutions
Application-specific delivery systems tailored to your unique ASO sequences and disease targets.
Expert Formulation
Advanced platforms for GalNAc conjugation, LNP formulation, and AOC development to ensure high yield and purity.
Comprehensive Support
From antibody discovery to IND-enabling studies, we handle every step of the pipeline with full technical reporting.
High Scalability
Seamless scale-up from milligram discovery batches to gram-scale pilot production with rigorous quality control.
Proprietary Linker Library
Access to exclusive cleavable and non-cleavable linkers optimized for diverse intracellular environments and organs.
Regulatory Compliance
All delivery studies are performed to research-grade and preclinical standards to support your translational readiness.
Frequently Asked Questions
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