Gene Editing Service
Accelerate Your Research with Precision Genome Editing! Are you currently facing challenges in creating precise disease models, validating drug targets, or engineering cell lines with specific genetic modifications? Creative Biolabs' Gene Editing Service helps you overcome these hurdles by providing tailored CRISPR/Cas9 solutions for generating precisely engineered cell lines, enabling you to accelerate your research and achieve groundbreaking discoveries through advanced recombinant DNA technology.
How Creative Biolabs' Gene Editing Service Can Assist Your Project?
Creative Biolabs' Gene Editing Service delivers precisely engineered cell lines tailored to your research needs. We offer comprehensive solutions, from initial consultation and design to final cell line validation.
Workflow
Our streamlined workflow ensures efficient and reliable generation of your desired cell lines.
- Required Starting Materials: Clients typically provide target gene sequence information, specific modification goals (e.g., knockout, knock-in, point mutation), and the desired cell line (if not using Creative Biolabs' cell lines).
- Key Steps Involved:
Project Consultation and Design
We begin with a detailed consultation to understand your research goals and design a customized and optimized gene editing strategy.
System Set-up and Validation
We construct the most optimal strategy (plasmids, lentivirus, or RNP) and validate their performance to ensure high efficiency of the strategy and specificity of sgRNA.
Cell Transfection
We introduce gene editing elements into your chosen cell line using optimized transfection methods showing high editing efficiency. We then apply drug selection strategies (if needed) or cell sorting (if needed) to enrich for successfully modified cells.
Single-Cell Cloning and Expansion
To obtain clonal cell lines with homogeneous or heterozygous gene editing, we perform single-cell cloning by limiting dilution, isolating and expanding individual cells to generate stable cell lines to ensure genetic uniformity.
Validation of Gene Modification
We rigorously validate the generated single clone cell lines to confirm the desired genetic modification by sequencing.
Add-ons
We also provide karyotyping service, functional test like WB and FACS, and so on. For iPSC-related projects, we also provide additional characterization services like pluripotency testing.
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Final Deliverables: Upon project completion, you will receive
- Fully characterized, validated, mycoplasma-free gene-edited single cell clones.
- A comprehensive project report detailing the experimental procedures, validation data (including sequencing), quality control information, and results of add-ons.
- Estimated Timeframe: The typical timeframe for this service ranges from 12 to 20 weeks, depending on the complexity of the gene editing project, the specific cell line used, and the type of modification required.
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Why Choose Us?
Creative Biolabs' Gene Editing Service stands out due to our commitment to quality, efficiency, and customer satisfaction. We leverage cutting-edge CRISPR/Cas9 technology, a team of experienced scientists, and a rigorous quality control process to deliver exceptional results.
- We offer end-to-end solutions, handling everything from experimental design to final cell line validation.
- Our proprietary technologies and optimized protocols ensure high efficiency and specificity.
- We provide comprehensive quality control, including Sanger sequencing, functional assays, and add-ons to guarantee the accuracy and reliability of our gene-edited cell lines.
- We have a proven track record of success, with a high success rate in generating complex gene edits.
Case Study
Creative Biolabs constructs the iPSC line with the knock-in of GFP. The single cell clone shows high GFP expression detected by FACS.
Fig.1 iPSC-GFP cell line constructed by CRISPR-mediated gene knock-in.
Customer Reviews
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Introduction of Gene Editing Service
Gene editing, particularly using CRISPR/Cas9 technology, has revolutionized biological research by enabling precise and targeted modifications to the genome. This technology has broad applications, including disease modeling, drug discovery, and gene therapy. CRISPR/Cas9 offers advantages in terms of efficiency, specificity, and versatility compared to earlier gene editing methods. Published data supports the wide adoption and significant impact of CRISPR/Cas9 technology in advancing scientific knowledge and developing new therapeutic strategies.
FAQs
How does Creative Biolabs ensure gene editing accuracy?
Creative Biolabs employs rigorous quality control, including Sanger sequencing and functional assays, to verify desired genetic modifications and ensure gene-edited cell line accuracy. We also perform off-target analysis. Contact our team for QC details.
What cell types are compatible with Creative Biolabs' Gene Editing Service?
Our service works with various cell types, including stem cells (iPSCs) and non-stem cell lines. We are also familiar with suspension cell editing. Contact us to discuss your cell line requirements.
What are CRISPR/Cas9's advantages?
CRISPR/Cas9 offers high efficiency, specificity, and ease of use, enabling precise targeting of specific DNA sequences for modifications like knockouts, knock-ins, and point mutations. Contact us to learn more.
What support does Creative Biolabs provide?
Creative Biolabs provides comprehensive support throughout the entire process, from initial consultation and design to final cell line validation. Our experienced scientists ensure your project's success. Contact us to discuss your needs.
Related Products
Creative Biolabs offers readily available, high-quality gene-edited iPSC cell lines:
| Products | Donor | Size | Quality control |
|---|---|---|---|
| GFP labeled iPSC cell line | Healthy | 106 cells / vial |
≥ 95% GFP+ cells
|
Related Services
Creative Biolabs offers a range of complementary services and products that can help you achieve your research goals. These include:
How to Contact Us
Creative Biolabs is dedicated to providing high-quality gene editing services to accelerate your research. Our expertise in CRISPR/Cas9 technology, combined with our commitment to customer satisfaction, ensures that you receive the best possible results.
Contact Our Team for More Information and to Discuss Your Project
