Custom dCas9-VPH Overexpressing Cell Line Development Service
A dCas9-VPH overexpressing cell line is a genetically engineered model that expresses dead Cas9 (dCas9) fused with the VPH transactivation domain, enabling precise gene activation without causing double-strand breaks. These cell lines are valuable tools for epigenetic regulation, gene function analysis, and transcriptional activation screening. By enabling targeted control of endogenous gene expression, they offer critical insights into gene networks and disease pathways. With strong expertise in CRISPR-based engineering, Creative Biolabs delivers customized and stable dCas9-VPH cell line development services to support diverse research and therapeutic applications.
Introduction of Custom dCas9-VPH Overexpressing Cell Line Development Service
The custom dCas9-VPH overexpressing cell line is a versatile tool for gene activation and functional genomics. It expresses dead Cas9 (dCas9)—a nuclease-inactive form of Cas9 that binds target DNA without cutting it. When fused to VPH, a synthetic activator combining VP64, p65, and HSF1, the dCas9-VPH complex enables targeted transcriptional activation of endogenous genes without genomic disruption. Establishing dCas9-VPH overexpressing cell lines enables researchers to conduct large-scale gain-of-function studies, transcriptional activation screens, and pathway elucidation. Moreover, they are compatible with a wide range of commercial CRISPR activation (CRISPRa) libraries and can also be tailored for custom sgRNA libraries targeting specific gene sets of interest, particularly valuable in studying gene regulatory networks and therapeutic target discovery.
Workflow of Custom dCas9-VPH Overexpressing Cell Line Development Service
Fig. 1 Workflow of our custom dCas9-VPH overexpressing cell line development service.
Advantages of Custom dCas9-VPH Overexpressing Cell Line Development Service
- Flexible Workflow but not simple - A customizable and flexible development process tailored to client needs, helping simplify downstream experiments and ensure smooth project progression.
- Strict Quality Control - Rigorous validation ensures stable integration and high-level expression of dCas9-VPH, guaranteeing reliable and reproducible gene activation performance.
- Broad Cell Type Compatibility - Compatible with a wide variety of cell types, including immortalized cell lines, stem cells, and primary cells, to meet diverse research needs.
- High-Throughput Ready - Fully supports large-scale functional screening using both commercial CRISPRa libraries and customized sgRNA libraries for targeted studies.
- Comprehensive Support - End-to-end technical assistance, timely communication, and dependable after-sales service ensure a seamless and satisfying customer experience.
How Custom dCas9-VPH Overexpressing Cell Line Development Service Can Assist Your Project
As part of our custom dCas9-VPH overexpressing cell line development service, Creative Biolabs delivers high-quality, mycoplasma-free monoclonal cell lines with robust and stable expression of dCas9-VPH. Each clone undergoes comprehensive multi-level validation, including genomic PCR, qPCR, Western blot, and expression/functionality assays to ensure high expression fidelity and transcriptional activation efficiency. These rigorously verified cell lines are ready-to-use and compatible with both commercial and custom CRISPRa libraries, enabling clients to conduct accurate gene activation, functional screening, and pathway analysis with confidence and reproducibility.
By minimizing technical variability and development time, Creative Biolabs helps accelerate your research and improves experimental outcomes. To discuss your project or request a quote, please get in touch with us today—we are here to support your success from concept to results.
