Custom dCas9-VPR Overexpressing Cell Line Development Service
The dCas9-VPR overexpressing cell line is a genetically engineered model where a nuclease-deactivated Cas9 (dCas9) is fused with the VPR transcriptional activator (VP64, p65, Rta). Guided by sgRNA, dCas9-VPR binds specific genomic sites to robustly activate target gene expression without cutting DNA. This system is widely applied in CRISPRa-related research for gene function analysis, drug screening, and disease modeling. Creative Biolabs offers customized, high-quality dCas9-VPR cell line development using trusted CRISPR technology. We ensure stable expression, high efficiency, and reproducibility—empowering clients to explore gene regulation and accelerate therapeutic discovery with confidence.
Introduction of Custom dCas9-VPR Overexpressing Cell Line Development Service
The dCas9-VPR overexpressing cell line is a powerful genetic tool for precise gene activation without causing DNA cleavage. dCas9 is a deactivated form of Cas9 that binds specific DNA sites without introducing double-strand breaks. When fused with VPR, a strong transcriptional activator composed of VP64, p65, and Rta, it forms a complex that can be directed by sgRNAs to target promoters and activate gene expression effectively. Unlike traditional CRISPR/Cas9 systems, dCas9 serves as a DNA-bound scaffold, recruiting the VPR module to drive transcription in a programmable, non-disruptive way. This makes it an ideal platform for CRISPRa-based research. Establishing a stable dCas9-VPR overexpressing cell line enables durable and consistent gene activation, supporting a wide range of applications such as functional genomics, pathway analysis, cell fate reprogramming, drug discovery, and disease modeling—all without altering the genome.
Workflow of Custom dCas9-VPR Overexpressing Cell Line Development Service
Fig. 1 Workflow of our custom dCas9-VPR overexpressing cell line development service.
Advantages of Custom dCas9-VPR Overexpressing Cell Line Development Service
- Wide Cell Type Compatibility - Efficiently applies dCas9-VPR across various cell types, including immortalized lines, stem cells, and primary cells, to meet different research demands.
- Flexible Yet Focused Workflow - Customizable development ensures smooth integration of dCas9-VPR, simplifying your downstream experiments.
- High-Throughput Capable - Designed for large-scale screening, supporting both commercial CRISPRa libraries and custom sgRNA sets.
- Stringent Quality Control - Validated for stable expression and reliable performance of dCas9-VPR, ensuring consistent gene activation.
- Comprehensive Technical Support - Full project support with expert guidance and responsive service, ensuring success at every step of your dCas9-VPR workflow.
How Custom dCas9-VPR Overexpressing Cell Line Development Service Can Assist Your Project
At Creative Biolabs, our custom dCas9-VPR overexpressing cell line development service is committed to delivering high-quality monoclonal cell lines tailored to your research needs. Each single-cell clone is rigorously screened and validated to ensure stable integration and high-level expression of dCas9-VPR. We perform comprehensive quality control procedures, including genomic PCR and RT-qPCR for integration verification and expression quantification, Western blotting to confirm protein expression levels, and mycoplasma testing to ensure cell line cleanliness and biosafety. These robust, mycoplasma-free monoclonal lines offer reliable and reproducible gene activation, significantly enhancing the accuracy and consistency of downstream applications such as functional genomics, drug screening, and pathway analysis - regardless of whether you are using commercial CRISPRa libraries or fully customized sgRNA libraries.
Let Creative Biolabs accelerate your research with dependable, validated cell line models, get in touch with us to discuss your custom dCas9-VPR project and receive expert support every step of the way.
